A number of different collections have been donated to the RHS Herbarium over the years. Some of the more significant collections are: Hanbury Herbarium, British Pteridological Soceity's Herbarium, Alpine Garden Society's Herbarium, The Heather Society's Herbarium, Steggall Herbarium, Harry Smith Collection, Rev. George Henslow, George Forrest, David McClintock and Ken Page.
The RHS Herbarium currently has no means of storing other reference material such as spirit collections or DNA samples.